Fig. 2: S-protein specific IgG1 repertoires are polyclonal and unique per donor, whereas within a donor substantial cross-reactivity is observed when enriching with the four VOC S-protein trimers.
From: Discriminating cross-reactivity in polyclonal IgG1 responses against SARS-CoV-2 variants of concern
a Quantitative overlap of IgG1 repertoires illustrated by a heatmap, depicting the degree of overlap between all detected IgG1 repertoires, i.e. from all donors, extracted from either the full plasma (FP) or after pulldowns with each of the VOCs S-protein of all variants studied (WT, Alpha, Beta and Gamma). The quantitative overlap of Fab molecules, based on intensity per each unique identifier RT#mass, was quantified and shown as a percentage as indicated by the color bar. The zoomed-in panels for donors 002 and 003 highlight the substantial overlap for donor 003 between the S-protein specific Fab profiles, and the full plasma IgG1 profile Source data are provided as a Source Data file. b Deconvoluted full Fab mass profiles as obtained for donor 002 and 003, from the S-protein specific Fab profiles (WT, Alpha, Beta and Gamma) and the full plasma Fab profile (top). Each peak represents a unique Fab at its detected mass and plasma concentration. The number on the right of each S-protein specific profile, indicated the y-axis multiplier compared to the y-axis used for the full plasma profiles.Supplementary Figs. 3–5 depict the full Fab mass profiles of all the other six donors.
