Fig. 3: Translation and ribosome biogenesis are downregulated in cells with increased ploidy.

a Quantification of rRNA abundance in cells of different ploidy. qRT-PCR was used in four independent experiments, the values were normalized to the expression of the housekeeping gene ACT1 and RPL30. Top: schematics of the primers’ localization. b Puromycin incorporation in cells of different ploidy. Two types of loading were applied: left: equal amount of protein lysates were loaded, right: lysates from equal cell number was loaded. Ponceau staining was used as a loading control. c, d Quantification of the relative puromycin incorporation calculated from three independent experiments; means with SEM are shown. e Sensitivity of cells of different ploidy to puromycin and rapamycin treatment. f Quantification of rRNA in haploid wt cells and in mutants lacking TUP1. The experiment was performed as in a. g Representative immunoblotting of puromycin incorporation in haploid wt and tup1∆ cells. h Quantification of puromycin incorporation from three independent biological replicates. Equal amounts of protein lysates were loaded, Ponceau staining was used as a loading control. All plots show means with SEM from at least three independent experiments, the statistical evaluation was performed using the unpaired, two-tailed t-test, the p values are included. ns not significant. Source data are provided as a Source Data file.