Fig. 4: Regulation of rRNA expression in polyploid cells via Tor1-Sch9-Tup1 signaling pathway.

a Relative abundance of Tup1 in cells of different ploidy compared to haploid cells treated with rapamycin. b Quantification of three independent experiments as in a. Mean with SEM is shown. c Representative immunoblot showing the abundance of Tup1 in wt and sch9∆ haploid cells with and without the TORC1 inhibitor rapamycin (Rapa). d Representative immunoblot showing the abundance of Tup1 in sch9-as haploid cells with and without the ATP analog 1NM-PP1, resolved on polyacrylamide gel (PAGE) with and without Phos-tag. Two different mutant clones were tested. e Quantification of the relative Tup1 abundance from d. Three independent experiments were performed, mean with SEM is shown. f qRT-PCR quantification of rRNA abundance in cells lacking Sch9 and Tup1, with and without rapamycin treatment. Means and SEM of three independent experiments are shown. g Representative immunoblotting of Sch9 on PAGE and Phos-TAG gel in cells of different ploidy. h Representative immunoblotting of Tup1 on PAGE and Phos-TAG gel in cells of different ploidy. i Representative immunoblotting of Tup1 after cyclohexamide shut off. Different time points were collected and equal amount of lysate was loaded. j Quantification of three independent experiments from h. Means and SEM are shown. Source data are provided as a Source Data file, p values of the two-tailed, unpaired Student’s t test are shown.