Fig. 2: Single-Cell Transcriptomics of Bone Marrow-derived Mononuclear Cells. | Nature Communications

Fig. 2: Single-Cell Transcriptomics of Bone Marrow-derived Mononuclear Cells.

From: Reprogramming of myeloid cells and their progenitors in patients with non-medullary thyroid carcinoma

Fig. 2

A UMAP showing 275 CD34+ progenitor cells derived from bone marrow tissue from 15 samples (HC n = 5, MNG n = 3, TC n = 7). Canonical markers were used to annotate the different cell types (B). B Dot heatmap showing the expression of cell type markers used to annotate the bone marrow-derived progenitor cell types. C GO term enrichment analysis of the cell type-specific markers for each of the progenitor cell populations. Over-representation analysis was used to determine significance. P values were measured using the hypergeometric distribution and adjusted using Benjamini-Hochberg correction. D Box plots of cell proportions within the CD34+ progenitor cell populations derived from the bone marrow in healthy controls (n = 5), MNG (n = 7) and TC (n = 3). The center line per box plot refers to the median, bounds are the 25th and 75th percentiles (interquartile range; IQR). Box plot whiskers are the smallest and largest values no further than 1.5*IQR from the box plot bounds. No significant differences were found between the conditions. E–G UMAP of the CD34+ progenitor cell populations, along with the PBMC-derived B cells. Colored by cell type, pseudo time and condition, respectively. H Volcano plots showing differential expression of (i) TC vs. HC, (ii) TC vs. MNG and (iii) MNG vs. HC within the myeloid multipotent progenitors. P values were obtained from the MAST test (two-sided) and adjusted using Bonferroni correction. I PCA plots showing the first two principal components of the pseudo-bulk RNAseq profile from the periphery (PBMC) and the bone marrow-derived mononuclear cells (BM-MNC). J Violin plots showing enrichment of the PBMC cell type-specific differentially expressed genes in BM-MNC cell populations (left), and vice-versa (right). Wilcoxon two-sided test between TC and HC for each cell type separately with Benjamini-Hochberg correction over all cell types for PBMCs and BM-MNCs separately. PBMCs p values <0.0001, BM-MNC p values <0.00001. Source data are provided as a Source Data file.

Back to article page