Fig. 4: Effect of treatment on functional phenotype of peripheral blood classical monocytes and circulating inflammatory mediators in peripheral blood plasma.

A Study Design Follow-up Measurements: Peripheral blood was collected from TC and MNG patients before treatment (baseline), 30 days after surgery, 7 days and 30 days after treatment with radioactive iodine. Illustration created with BioRender.com. B Differential cell counts in whole blood. (HC n = 8, MNG n = 4–13, TC n = 8–13). Mean ± SEM. Mixed effects model (repeated measures two-way ANOVA with missing values) followed by Bonferroni correction. Each time point was compared to the baseline time point, as not all study participants received both treatments. Adjusted p values: MNG baseline vs. 7d post I131 p = 0.011, baseline vs. 30d post I131 p = 0.003; TC baseline vs. 7d post I131 p = 0.034. C Cytokine release of CD14⁺ CD16^- classical monocytes after 24 h stimulation with TLR4 ligand LPS. (HC n = 7; MNG: baseline n = 13, 30d post-surgery n = 4, 7d post I131 n = 6, 30d post I131 n = 5; TC: baseline n = 13, 30d post-surgery n = 13, 7d post I131 n = 8, 30d post I131 n = 7). Median with IQR and Tukey whiskers. Corrected for age and sex. Mixed effects model (repeated measures two-way ANOVA with missing values) followed by Bonferroni correction. Each time point was compared to the baseline time point, as not all study participants received both treatments. D ROS production of CD14⁺ CD16⁻ classical monocytes after 24 h stimulation with TLR4 ligand LPS. (HC n = 7, MNG: baseline n = 8, 30d post-surgery n = 2, 7d post I131 n = 5, 30d post I131 n = 4; TC: baseline n = 7, 30d post-surgery n = 9, 7d post I131 n = 6, 30d post I131 n = 4) Median with IQR and Tukey whiskers. Two-way ANOVA followed by Bonferroni correction. Corrected for age and sex. Source data are provided as a Source Data file.