Fig. 1: LCOs and COs activate calcium oscillations in barley.
From: Nutrient regulation of lipochitooligosaccharide recognition in plants via NSP1 and NSP2
a Representative calcium traces of atrichoblasts of barley (H. vulgare) lateral roots grown in nutrient depletion, responding to 10−8 M CO4, 10−8 M CO8, 10−7 M NS-LCO (non-sulphated LCO produced by R. irregularis), 10−7 M SmLCO (LCO produced by S. meliloti), and 10−7 M MlLCO (LCO produced by Mesorhizobium loti). Numbers indicate cells responding compared to total cells analyzed. Dose-response curves for LCO and CO induction of calcium oscillations in barley (b) and M. truncatula (c) lateral roots. Calcium spiking (%) indicates the ratio of cells responding with calcium oscillations. d AMF colonization measured at 7 weeks post-inoculation of barley wild type (WT), symrk, ccamk, cyclops, and ram1 mutants, with the inset showing representative traces of barley symrk mutant atrichoblasts responding to 10−7 M SmLCO and 10−7 M CO4. Numbers indicate cells responding compared to total cells analyzed. n = 3-5 biologically independent plants. Total: total colonization; A: arbuscules. For statistical analysis a one-sided Wilcoxon test was performed. **p < 0.01.
