Fig. 2: IAV PB2 promotes the degradation of JAK1.
From: The influenza virus PB2 protein evades antiviral innate immunity by inhibiting JAK1/STAT signalling
a Immunoblots of HEK293T cells were transfected with different concentrations of PB2 plasmids from PR8 (left) or CZ (right). b, c Immunoblots of HEK293T cells transfected with JAK1-His (b) or Flag-STAT1 (c) with different concentrations of PB2 plasmids. d qPCR analysis of JAK1 mRNA level in A549 cells transfected with different concentrations of PB2 plasmids (n = 3 biologically independent samples). Data are presented as the mean ± SD. Statistical significance was determined by unpaired two-tailed Student’s t test. ns P > 0.05. e Immunoblots of A549 cells infected with PR8 (left) or CZ (right) at an MOI of 1. f Immunoblots of stable PB2 knockdown HEK293T cells infected with PR8 virus (MOI = 0.1). g Immunoblots of HEK293T cells were transfected with PB2-PR8 and treated with CHX. h Immunoblots of HEK293T cells were transfected with the PB2 plasmids and treated with DMSO, MG132, NH4Cl, or chloroquine (CQ). Data are one representative of three independent experiments.
