Fig. 3: IAV PB2 protein increases the K48-linked ubiquitination of JAK1.
From: The influenza virus PB2 protein evades antiviral innate immunity by inhibiting JAK1/STAT signalling
a Ni-NTA pull-down analysis of the ubiquitination of JAK1 in HEK293T cells transfected with JAK1-His, HA-ubiquitin (HA-Ub), and PB2 plasmids and treated with MG132. b Ni-NTA pull-down analysis of the ubiquitination of JAK1 in HEK293T cells transfected with JAK1-His, HA-Ub, or its mutants K48 and K63 [K at indicated residue, and K at other residues were simultaneously mutated to arginines], and PB2 plasmids and treated with MG132. c Co-IP analysis of the ubiquitination of JAK1 in A549 cells infected with PR8 or CZ at an MOI of 0.01. d Co-ip analysis of the ubiquitination of JAK1 in stable PB2 knockdown HEK293T cells infected with PR8 virus. WCL, whole-cell lysates. Data are one representative of three independent experiments.
