Fig. 4: PB2-mediated JAK1 degradation at residues 859K and 860K.
From: The influenza virus PB2 protein evades antiviral innate immunity by inhibiting JAK1/STAT signalling
a Schematic representation of the deletion mutants of JAK1 (upper) and immunoblots of HEK293T cells transfected with JAK1 or its deletion mutants and PB2 plasmids (lower). b Ubiquitination modification online prediction of JAK1 by CPLM 1.0. c Immunoblots of HEK293T cells transfected with JAK1 or its mutants and PB2 plasmids. d Intensities analysis of the bands on the immunoblots (c) from three independent experiments. Data are presented as the mean ± SD and statistical significance was determined by unpaired two-tailed Student’s t test. ns P > 0.05. e Immunoblots of HEK293T cells transfected with JAK1 or its mutants and PB2 plasmids. f Molecular model of the JAK1 kinase domain generated by PyMOL (PDB: 4ehz). Residues K859/860 are highlighted in pink. g Ni-NTA pull-down analysis of the ubiquitination of JAK1 in HEK293T cells transfected with JAK1 or its mutants, HA-Ub and PB2 plasmids, and treated with MG132. WCL, whole-cell lysates. Data are one representative of three independent experiments.
