Fig. 6: JAK1 interacts with IAV PB2.
From: The influenza virus PB2 protein evades antiviral innate immunity by inhibiting JAK1/STAT signalling
a Co-ip and Ni-NTA pull-down analysis of the interaction of PB2 with JAK1 in HEK293T cells transfected PB2 and JAK1 plasmids. b Co-ip and Ni-NTA pull-down analysis of the interaction of PB2 with JAK1 in HEK293T cells transfected with JAK1 plasmids and infected with PR8 or CZ virus. c Colocalization of endogenous JAK1 (green) and PB2 (red) in PR8 or CZ-infected A549 cells. Nuclei were stained with DAPI (blue). Scale bars, 10 ÎĽm. Intensities of fluorescence at indicated locations were scanned by LAS X Software. d Schematic representation of the deletion mutants of PB2. e, f Ni-NTA pull-down (e) and Co-ip analysis (f) of the interaction of JAK1 with PB2 and its truncation mutants in HEK293T cells. WCL whole-cell lysates, HC Heavy Chain, LC Light Chain. Data are one representative of three independent experiments.
