Fig. 2: Synthesis, characterization, and hypoxia responsiveness of LacAC4A. | Nature Communications

Fig. 2: Synthesis, characterization, and hypoxia responsiveness of LacAC4A.

From: Lactose azocalixarene drug delivery system for the treatment of multidrug-resistant pseudomonas aeruginosa infected diabetic ulcer

Fig. 2

a Synthetic route of LacAC4A: (i) NaNO2, HCl, 0–5 °C; (ii) Propargyl amine, 2-(7-azabenzotriazol-1-yl)-N,N,N’,N’-tetramethyluronium hexafluorophosphate, N,N-diisopropylethylamine, rt; (iii) Lac-N3, tetrahydrofuran/H2O, CuSO4·5H2O, L-ascorbic acid sodium salt, 55 °C; (iv) CH3OH, CH3ONa, rt; b The reduction reaction of LacAC4A. c CAC of LacAC4A. d DLS result of LacAC4A (0.10 mM). e TEM image of LacAC4A. Scale bar was 20 nm. f Absorbance spectra of LacAC4A (10 μM) before and after reducing by SDT (10 mM). g Absorbance at 420 nm of LacAC4A (10 μM) as a function of time following the addition of SDT (10 mM). h Fluorescence intensity at 575 nm of RhB@LacAC4A (2.0/20 μM) as a function of time following the addition of SDT (10 mM). The experiments of f-h were carried out in PBS (10 mM, pH = 7.4) at 37 °C.

Back to article page