Fig. 2: Microglial debris is unlikely to be engulfed by pericytes, endothelial cells, VSMCs, OPCs, oligodendrocytes, NSCs, neurons or circulating blood cells in vivo. | Nature Communications

Fig. 2: Microglial debris is unlikely to be engulfed by pericytes, endothelial cells, VSMCs, OPCs, oligodendrocytes, NSCs, neurons or circulating blood cells in vivo.

From: Microglial debris is cleared by astrocytes via C4b-facilitated phagocytosis and degraded via RUBICON-dependent noncanonical autophagy in mice

Fig. 2

a Scheme of in vivo debris engulfment examinations by microglial depletion. b Microglial debris is unlikely to be phagocytosed by pericytes, endothelial cells, VSMCs, OPCs, oligodendrocytes, neurons or NSCs in vivo. NESTIN-GFP is illustrated by the red pseudocolor for better visualization. Astrocytes (GFAP): 0.24 ± 0.05%, pericytes (PDGFR-β): 0.03 ± 0.01%, endothelial cells (CD31): 0.03 ± 0.01%, VSMCs (α-SMA): 0.03 ± 0.02%, OPCs (PDGFR-α): 0.08 ± 0.02%, oligodendrocytes (MBP): 0.05 ± 0.01%, oligodendrocytes (CC1): 0.07 ± 0.02%, neurons (TUJ1): 0.04 ± 0.01%, NSCs (NESTIN-GFP): 0.03 ± 0.01%. N = 4 mice in the NESTIN-GFP group and N = 5 mice in the rest of the groups. One-way ANOVA with Holm‒Sidak’s multiple comparisons test (post hoc). P = 3.1086e–015, 4.6629e–015, 2.6645e–015, 1.0612e–012, 3.4639e–014, 9.7167e–013, 4.9960e–015, and 1.2434e–014. c Scheme of the in vivo BBB integrity examination with 10-kDa dextran-FITC. d Confocal images show that the BBB is not compromised during microglial depletion in vivo. Each experiment was independently repeated from 4 mice with similar results. PLX5622: PLX5622-formulated AIN-76A diet; VSMCs vascular smooth muscle cells, OPC oligodendrocyte precursor cell, OL oligodendrocyte, NSC neural stem cell, IV intravenous. The source data are provided as a Source Data file.

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