Fig. 9: Microglial debris-containing LAPosomes are fused with acidic lysosomes for degradation in vivo and in vitro. | Nature Communications

Fig. 9: Microglial debris-containing LAPosomes are fused with acidic lysosomes for degradation in vivo and in vitro.

From: Microglial debris is cleared by astrocytes via C4b-facilitated phagocytosis and degraded via RUBICON-dependent noncanonical autophagy in mice

Fig. 9

a Scheme of the in vivo examination of astrocytic engulfment involving AAV PHP.eB-based astrocyte labeling and microglial depletion. b Intra-astrocytic microglial debris colocalized with LAMP1 in vivo. Each experiment was independently repeated from 7 mice with similar results. c Schemes of the in vitro astrocytic engulfment assay using pHrodo-labeled microglial debris. d pHrodo-labeled microglial debris localized with LAMP1 in in vitro cultured astrocytes. Each experiment was independently repeated from 4 biological replicates with similar results. e The degradative product (lipid droplets) is reduced after disruption of the formation of phagolysosomes in vitro by chloroquine. D3: 1.00 ± 0.09, D7 w/o CQ: 2.51 ± 0.51, D7 w/ CQ: 0.66 ± 0.18. N = 3 biological replicates of each group. One-way ANOVA with Holm‒Sidak’s multiple comparisons test (post hoc). PLX5622: PLX5622-formulated AIN-76A diet; CD control AIN-76A diet, IV intravenous. Data are presented as mean ± SD. The source data are provided as a Source Data file.

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