Fig. 1: Single-cell sequencing reveals the diverse functional states of glioma cells post-radiation. | Nature Communications

Fig. 1: Single-cell sequencing reveals the diverse functional states of glioma cells post-radiation.

From: P300 promotes tumor recurrence by regulating radiation-induced conversion of glioma stem cells to vascular-like cells

Fig. 1

a UMAP plot of cell clusters from control (0 Gy) and 2- and 7-days radiated (8 Gy) fractions. b Histogram shows the number of cells per cluster in each group. c Heatmap shows Louvain clustering of co-expressed gene network modules and EnrichR GO_terms associated with each cluster. Each subgroup is highlighted by a black box. d Pseudotime trajectory analysis, subgroups are highlighted in colored circles and their functional cell states indicated in the inset. e Heatmap of average expression of markers of proliferation, glioma stem cells (GSC), Neural crest-mesenchymal stem cells (NC-MSC), pericytes, mesodermal and endothelial cells. f Heatmap of normalized enrichment scores (NES) of gene sets in glioma cells 2- and 7-days post-radiation. N = 3 biological replicates and only gene sets with p < 0.05 are shown. g Heatmap of relative expression of endothelial, pericyte and stemness genes in control and radiated cells. N = 3 independent experiments. *, ** indicates p < 0.05, p < 0.005, unpaired two-tailed t-test.

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