Fig. 4: iGEC and iGPC provide trophic support to promote tumor growth post-radiation.
a Schematic of expansion of iGEC and iGPC for in vitro conditioned media (CM) and in vivo co-transplantation tumor growth experiments. b Proliferation of control and radiated tumor cells. Error bars represent mean ± SD, N = 3 biological replicates, ** and *** indicates p < 0.001 and p < 0.0005, one-way ANOVA and post hoc t-test. c LogFc expression (FDR adjusted, p < 0.05) of trophic factors expressed by radiated (iGEC/iGPC) and nonradiated (nGEC/nGPC) and tumor cells. d Proliferation of sorted, nonradiated (0 Gy mC-) and radiated (8 Gy mC-) tumor cells 3-days post-treatment with recombinant factors. N = 3 biological replicates, * and ** indicates p < 0.05 and p < 0.005, one-way ANOVA. e, f Images of mice showing tumor growth at 2 and 4 weeks. Box plots show quantitation of tumor growth by luminescence in each group. Box plots display median, 25th and 75th percentile, and whiskers extend min and max values. N = 5 mice, * indicates p < 0.05, one-way ANOVA and post hoc t-test. g Kaplan-Meier survival curve of mice transplanted with tumor cells and GEC/GPC. N = 5 mice, and *, ** indicates p < 0.05, p < 0.005, Log-rank test. h Schematic outlines the depletion of GEC/GPC using HSV-DTK method in tumor xenografts i, j Images of mice showing tumor growth at 2 and 4 weeks. Box plots show the quantitation of tumor growth. N = 5 mice, *** indicates p < 0.005, one-way ANOVA and post-hoc t-test.
