Fig. 3: Mating pathway activation and changed morphology correlate.
From: Engineered cell differentiation and sexual reproduction in probiotic and mating yeasts
A–C Increased side-scatter area (SSC-A) correlates with median fluorescence intensities (MFI) from PFUS1-GFP reporter expression across cognate ligand dosages ranging 0–100 µM in A2bR, MT1, 5-HT4b, and Mam2 biosensing strains of S. cerevisiae (CPK153-161 and CPK165-167). Linear regression was performed using Graphpad Prism, and R2 is reported for each analysis. A All data (n = 216). B Highlights and correlation of each Gα expression design in all data (n = 72 × 3). C Individual representation of correlation for all hGPCR designs (n = 54 × 4). D Representative morphologies in S. cerevisiae strains from panels A–C in the absence (−ligand) or presence of cognate ligands (+ligand, 100 µM) for A2bR, MT1, and 5-HT4b, or Mam2 (1 µM). The experiment was repeated more than three times. Size bars illustrate 10 µm. E Increasing SSC-A for each strain design in panels A–C across 0–100 µM cognate ligand supplementation as indicated. SSC-A is normalized for all strains to the PPGK1-Gα strain without ligand supplementation within each hGPCR design. Data represent means and standard deviations from three biological replicates. Source data are provided as a Source Data file.
