Fig. 2: Liver-specific deletion of Prom1 in mice impairs liver regeneration after partial hepatectomy.
From: Central role of Prominin-1 in lipid rafts during liver regeneration
A 2/3 partial hepatectomy was performed in 8-week-old male Prom1f/f and Prom1LKO mice. a Ratio of liver-to-body weight on the indicated days after PHx (n = 4 for sham, 12 h, and 120 h, n = 5 for 24 h, n = 7 for 48 h, p = 0.034 for 48 h, p = 0.005 for 120 h). b The relative mRNA levels of cell cycle genes (Ccnd, Ccne, Ccnb) 48 hours after PHx (n = 8). Each mRNA level was normalized by 18 S rRNA (p = 0.041 for Ccnb, p = 0.029 for Ccnd, p = 0.039 for Ccne). c Immunoblotting for PROM1 and cell cycle proteins (Cyclin A, B, and E, and PCNA) 48 hours after PHx. d Statistical analysis of the band intensity in c (n = 3 independent samples, p = 0.032 for Cyclin A, p = 4.508 × 10−5 for Cyclin B, p = 0.042 for Cyclin E, p = 0.049 for PCNA). The band intensity of each protein was normalized to that of β-actin. e Representative H&E staining in the liver 48 hours after PHx. Mitotic cells are indicated by arrows. The experiment was repeated independently three times with similar results. f Representative double immunofluorescence for Ki-67 and HNF4α in the liver 48 hours after PHx. g Statistical analysis of the number of Ki-67-expressing cells after PHx (n = 3, p = 1.149 × 10−6). The number of Ki-67-positive cells was normalized to the number of DAPI-stained dots. Scale bar = 100 µm. Two-sided student t-test; *p < 0.05, **p < 0.01, ***p < 0.001. Data are expressed as the mean ± SEM with individual values. Source data are provided as a Source Data file.
