Table 1 Enzymatic activity of WbbBGT99 active site variants

From: The retaining β-Kdo glycosyltransferase WbbB uses a double-displacement mechanism with an intermediate adduct rearrangement step

WbbBGT99 variant

Relative turnover

wt

1a

R12A

0.029 ± 0.0054c

W20A

0.032 ± 0.0042b

W54A

0.012 ± 0.0017c

E158Q

N.D.d

R163A

0.089 ± 0.0022b

D232C

N.D.d

D232N

0.0038 ± 0.0021c

  1. aVariant evaluated at 0.5 μg/ml, with four replicates.
  2. bVariants evaluated at 5 μg/ml, with four replicates.
  3. cVariants evaluated at 50 μg/ml, with five replicates.
  4. dN.D.—not detected; assays results were indistinguishable from a no enzyme control (0.003 ± 0.003) at 50 μg/ml.
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