Fig. 4: Comparisons of prestin structures.
a Structural comparison of hPres-Cl− (PDB ID: 7LGU), PresTS-Cl−, hPres-Sal (PDB ID: 7LH2), PresTS-Sal, PresTS-SO42−, SLC26A9 (PDB ID: 6RTC), and AE1 (PDB ID: 4YZF). The models are superimposed with respect to the gate domain. The red box indicates the location of TM3 and 10. b Comparison of the structures of TMs 3 and 10 with those from other species (a). c Schematic diagram showing the relative positions of TM3 and TM10 within the membrane found in the prestin structures reported herein and by others. The structures are superimposed at the gate domain, and the TM3/10 positions are illustrated based on the centrally located S398. d Structural comparison of PresTS and hPres. The models are superimposed at the gate domain, and the TM3/10 positions are illustrated based on the centrally located S398. The Cl−-bound (left panel) and salicylate-bound (right panel) states are shown. The displacement distance of TM3/10 between PresTS and hPres was determined using S398 (in TM10) as the reference point. The structures are superimposed at the gate domain, and the TM3/10 positions are illustrated based on the centrally located S398.
