Fig. 1: Characterization and MAS NMR spectra of KIF5B bound to MTs. | Nature Communications

Fig. 1: Characterization and MAS NMR spectra of KIF5B bound to MTs.

From: Magic-angle-spinning NMR structure of the kinesin-1 motor domain assembled with microtubules reveals the elusive neck linker orientation

Fig. 1

a TEM images of the NMR sample before (left, middle) and after (right) MAS NMR experiments. Similar results were reproduced in three independent experiments. b SDS-PAGE  of the KIF5B/MT complex. The co-sedimentation assay conditions are detailed in Methods. This experiment was replicated three times with similar results. c Characteristic Ala (top left), Ser/Thr (middle left), Gly (bottom left) and aromatic (right) regions of the CORD and NCACX spectra of U-13C,15N-KIF5B bound to MTs. d 2D 1H-15N HETCOR spectra of U-2H,13C,15N-KIF5B bound to MTs. e Backbone walk for the A260-I265 stretch extracted from 3D NCACX (purple) and NCOCX (gray) spectra of U-13C,15N-KIF5B bound to MTs. Selected assignments are labeled in each spectrum. Source data are provided as a Source Data file.

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