Fig. 6: The 4T1-IE2 clone can directly suppress cytotoxic T cell activation and prevent further T cell activation by anti-PD1 immunotherapy. | Nature Communications

Fig. 6: The 4T1-IE2 clone can directly suppress cytotoxic T cell activation and prevent further T cell activation by anti-PD1 immunotherapy.

From: DNA barcoding reveals ongoing immunoediting of clonal cancer populations during metastatic progression and immunotherapy response

Fig. 6

A Measurement of intracellular IFN-gamma (IGNγ) production by 4T1-specific CD8 + T cells following co-culture with no 4T1 cells (No 4T1), bulk 4T1 cells (Bulk 4T1) and 4T1 clonal populations (4T1-NT1; 4T1-NT2; 4T1-IE1; 4T1-IE2). Four independent biological replicates in NT1 and NT2 groups, three independent biological replicates in IE1 and IE2 groups and one replicate for no 4T1 and bulk 4T1 groups. Data are shown as mean ± SEM. B Measurement of intracellular IFN-gamma production by 4T1-specific CD8 + T cells following co-culture with clonal 4T1 populations with or without anti-PD1 (PD1) or an isotype control (iso). Three biological replicates in IE1 and IE2 groups and four biological replicates in NT1 and NT2 groups. P value calculated using flow cytometry proportions fitted to a GLM with Bonferroni post hoc correction for multiple comparisons. Data are shown as mean ± SEM. Source data are provided as a source data file.

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