Fig. 4: Stool microbial diversity and transmission of Lachnospiraceae and Ruminococcaceae constituents.

A Shannon diversity index of stool microbiota showed lower alpha-diversity in pre-FMT versus post-FMT even before alcohol exposure. Alcohol exposure further worsened this in both groups. Pre Pre-FMT but not exposed to alcohol, Post Post-FMT but not exposed to alcohol, PreAlc Pre-FMT after alcohol exposure, PostAlc Post-FMT after alcohol exposure, **p < 0.01, *p < 0.05, Mann–Whitney for unpaired and Wilcoxon signed rank test for paired analyses (before/after alcohol) one-sided. There were 14 mice per group. Data presented as median, 95% CI (boxplot) with individual values and the entire range in the error bars. B Ruminococcaceae and C Lachnospiraceae genera heatmap of average relative abundance from human donors pre and post to GF mice. DonorPre: combined stools from patients with cirrhosis who were actively drinking that were collected before the FMT from a healthy human, DonorPost combined stools from the same patients with cirrhosis in DonorPre collected 15 days after the FMT from a healthy human, Pre-FMT stools from germ-free mice colonized with stools from DonorPre, Post-FMT stools from germ-free mice colonized with stools from DonorPost. Higher relative abundance of several genera belonging to Ruminococcaceae and Lachnospiraceae in DonorPost vs DonorPre, which was reflected in their respective mouse recipients.