Fig. 1: Expression pattern of TYK2, a T1D candidate gene, during human pancreatic development.
From: The type 1 diabetes gene TYK2 regulates β-cell development and its responses to interferon-α
a Schematic representation of the pancreatic differentiation protocol. The figure was partly generated using Servier Medical Art. b Heatmap of pancreatic lineage genes from deep RNAseq analysis at Stage (S) 1 (definitive endoderm), S4 (pancreatic-progenitors), and S7 (SC-islets). c Similar heatmap for known T1D candidate genes. Each gene is shown with a multiple testing corrected p value generated for the longitudinal differential expression of the gene during differentiation (n = 5). d Relative expression of TYK2 during pancreatic differentiation shown by qRT-PCR (n = 6) and e by immunoblot analysis for TYK2 protein. Tubulin was used as a loading control (n = 3). f Expression pattern of TYK2 in human fetal pancreas samples at 40 to 70 days post conception (n = 16). For d and f, significance was determined using one-way ANOVA with Tukey’s multiple comparison test, box and whiskers plots showing the median with whiskers extending from minimum to maximum values. *p < 0.05; **p < 0.01; ***p < 0.001. Source data are provided as a Source Data file.
