Fig. 5: TYK2 regulates the IFNα responses in SC-islets.
From: The type 1 diabetes gene TYK2 regulates β-cell development and its responses to interferon-α
a Immunoblot analysis for the phosphorylation of STAT1, STAT2, and STAT3 in Stage (S) 6 SC-islets in response to either IFNα or IFNγ treatment (n = 3). b Heatmap showing the differentially expressed genes in the WT (3348 cells), WT + IFNα (5202 cells), KO (4550 cells), and KO + IFNα (4281 cells) samples. Single-cell transcriptomics performed following 24 h IFNα (100 ng/ml) treatment on the WT and TYK2 KO S6 SC-islets. c Volcano plot showing the significant upregulated (soft Red), downregulated (iris blue) and non-significant (black) genes in response to IFNα treatment in β-like cells and α-like cells. d GSEA (KEGG) for selected enriched gene sets shown in α- and β- like cells in response to IFNα treatment. e Violin plots showing the normalized expression of Constitutive proteasome genes, Immunoproteasome genes, Transporter associated with antigen processing genes and f MHC Class Ι genes in S6 β- like cells in response to IFNα. Source data are provided as a Source Data file.
