Fig. 2: RNA methyltransferase METTL16 regulates erythroid differentiation in vivo.

a Mettl16 mRNA expression levels in each hematopoietic compartment (n = 3 technical replicates). HSC hematopoietic stem cells, MPP multipotent progenitors, CMP common myeloid progenitors, GMP granulocyte-monocyte progenitors, MEP megakaryocyte-erythrocyte progenitors. b Gene expression patterns of Mettl16 mRNA during erythroid differentiation generated from the single-cell RNA-seq analysis⁴⁸. c Survival rates of mice with each genotype. d Fetuses on E12.5 of control and Mettl16^fl/flEpor-Cre⁺ mice. e–g Flow cytometric analysis of the E10.5 peripheral blood from Mettl16^fl/flEpor-Cre⁺ mice (e). The histogram of CD71 intensity (f) and the relative CD71 mean fluorescence intensity (MFI) in the gated population (g, n = 4–5 mice). Data were pooled from two independent experiments. h Flow cytometric analysis of the E11.5 FL from Mettl16^fl/flEpor-Cre⁺ mice (n = 4–5 mice). i May-Grünwald-Giemsa stain of cytospin slides of E11.5 FL cells from Mettl16^fl/flEpor-Cre⁺ mice. j The histogram of FSC intensity in each population in h. k Flow cytometric analysis of the E12.5 FL from Mettl16^fl/flEpor-Cre⁺ mice. Data are expressed as mean ± SD (a, g, h). The p-values were calculated using two-tailed Student’s t-test (g, h). Images are representative of samples obtained from at least three mice (d and i–k). Source data are provided as a Source Data file.