Fig. 3: A network of stress responses is activated in BRCA-mut stroma. | Nature Communications

Fig. 3: A network of stress responses is activated in BRCA-mut stroma.

From: BRCA mutational status shapes the stromal microenvironment of pancreatic cancer linking clusterin expression in cancer associated fibroblasts with HSF1 signaling

Fig. 3

FFPE tumor sections from BRCA-mut and BRCA-WT PDAC patients were stained by MxIF using antibodies for the indicated proteins. (a, b) Representative images are shown. DAPI was used to stain nuclei. Scale bar, 50 μm. (c) Quantification of HSF1 mean intensity within all stromal cells in BRCA-mut (n = 14) and BRCA-WT samples (n = 20). 3-5 images per patient were analyzed using ImageJ software, HSF1 staining intensity was averaged within patients, and is presented as mean (across patients) ± SEM. Statistical analysis was conducted with a two-tailed unpaired t-test. (d, e) Pearson correlation matrices of stress TF coactivation in BRCA-mut (n = 8 for d; n = 13 for e) vs. BRCA-WT (n = 11 for d; n = 17 for e) patients. Source data are provided as a Source Data file.

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