Fig. 3: Changes in metabolite abundance induced by chronic hyperglycaemia. | Nature Communications

Fig. 3: Changes in metabolite abundance induced by chronic hyperglycaemia.

From: Altered glycolysis triggers impaired mitochondrial metabolism and mTORC1 activation in diabetic β-cells

Fig. 3

a Abundances of selected glycolytic, pentose phosphate pathway and TCA cycle metabolites in control (black bars) and diabetic (red bars) islets stimulated with 2 mM or 20 mM glucose. (n = 4 animals/genotype). Metabolites were measured by mass spectrometry, except for DHAP which was measured with a fluorimetric assay. b Schematic of glycolysis. c–f Activity of the indicated glycolytic enzymes in control (black) and diabetic (red) islets stimulated with 2 or 20 mM glucose. c Phosphofructokinase (PFK, n = 3 animals/genotype). d Fructose-1,6-bisphosphatase (FBPase, n = 3 animals/genotype). e Aldolase (n = 4 animals/genotype). f Glyceraldehyde-3-phosphate dehydrogenase (GAPDH, n = 3 animals/genotype). All panels show individual data points and mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed unpaired Student’s t test. Source data are provided as a Source Data file, and relative abundances of all metabolites identified are provided as Supplementary Data file 1.

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