Fig. 2: Functional genes in metatranscriptomes and metagenomes.

Variations across experiments and treatments in (a) transcript abundance (normalized as % of DNA recombination protein (recA) transcripts in metatranscriptomes) and (b) gene abundance (normalized as % of recA genes in metagenomes) of key functional genes. Functional genes quantified include those involved in: methane oxidation (particulate methane monooxygenase; pmoA), methanogenesis (methyl coenzyme M reductase; mcrA), phosphonate assimilation (C-P lyase and phosphonate-binding protein; phnJ and phnD), and phosphonate synthesis (phosphoenolpyruvate mutase and phosphonopyruvate decarboxylase; pepM and ppd). Abbreviations denote the experiment (e.g., UC4), the treatment (control, addition of the methanogenesis inhibitor 2-bromoethanesulphonate [BES], or high light intensity), and the sampling time point (t0 indicates initial sampling time point and tf indicates endpoint sample). Note the difference in the vertical axis for the LG5 experiment in comparison with the other experiments. Source data are provided as a Source Data file.