Table 1 In vivo activities of mutants

WT

100%

Yes

Negative control

0%

No

CcmA mutants

N36A^A

0%

Yes

K40A^A

0%

Yes

T41A^A

0%

Yes

H81A^A

82.7% ± 5.7%

Yes

D151A^A

0%

Yes

H184A^A

0%

Yes

CcmB mutants

H17A^B

61.9% ± 16.5%

Yes

F27H^B

46.7% ± 14.3%

Yes

F70A^B

47.3% ± 7.3%

Yes

D73A^B

58.9% ± 7.4%

Yes

D76A^B

39.8% ± 4.9%

Yes

L79A^B

19.2% ± 4.4%

Yes

H100A^B

46.7% ± 13.5%

Yes

H100Y^B

50.5% ± 6.5%

Yes

H186A^B

52.5% ± 15.4%

Yes

CcmC mutants

H60A^C

0%

No

R128A^C

31.1% ± 8.2%

low

R128D^C

0%

No

R128K^C

79.2% ± 9.0%

Yes

H184A^C

0%

No

H147A^C

58.0% ± 7.4%

Yes

H174A^C

55.1% ± 10.1%

Yes

ΔG186^C

56.8% ± 18.9%

Yes

G186A^C

74.6% ± 4.3%

Yes

ΔS187^C

0%

low

S187A^C

65.9% ± 14.0%

Yes