Fig. 7: Prognostic value of ISG15+CD8+ T cells. | Nature Communications

Fig. 7: Prognostic value of ISG15+CD8+ T cells.

From: Single-cell profiling reveals distinct adaptive immune hallmarks in MDA5+ dermatomyositis with therapeutic implications

Fig. 7

a Representative flow cytometric plots showing the gating strategies for ISG15+ B cells, and CD4+ and CD8+ T cells. Mouse CD3+ T cells were used as the negative controls. b Scatter plots showing the frequencies of ISG15+%B, ISG15+%CD4, ISG15+%CD8, MX1+%B, MX1+%CD4, and MX1+%CD8 cells from the indicated groups. The number of each group is indicated in parentheses. Data are presented as mean ± SD and the error bars denote SD. Statistical significance is calculated using the two-tailed Mann–Whitney test. c Univariate and multivariate Cox proportional hazards analyses of the indicated parameters for 1-year survival in active MDA5+ DM patients (n = 31). d Receiver operating characteristic (ROC) analysis of the indicated parameters to predict 1-year survival in active MDA5+ DM patients (n = 31). e Kaplan–Meier analysis of 1-year survival for ISG15+%CD8 and ferritin in MDA5+ DM patients (n = 31). The log-rank test is used. f Kaplan–Meier analysis of 6-month survival to compare the therapeutic efficacies in MDA5+ DM patients between the CNI group (n = 22) and the CNI + TOFA group (n = 14). The log-rank test is used. CNI, calcineurin inhibitor; TOFA, tofacitinib; ISG, interferon-stimulating gene. Source data are provided as a Source Data file.

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