Fig. 1: Alpha globin is the only globin expressed in the mouse endothelium.

a The mouse thoracodorsal artery (a skeletal muscle-derived resistance artery) shows endothelial expression of alpha globin, but not of other globins. Cytoglobin and neuroglobin are observed in adventitial layers, but not in endothelial cells. The scale bar represents 50 μm; magnified views focused on the endothelium are shown in the bottom right of each image. b The mouse aorta is representative of globin expression in a conduit artery. Only myoglobin is expressed in the aorta wall, and it is not expressed in endothelium. In all images, green signal is autofluorescence of the internal elastic lamina layer (IEL) and the asterisk indicates the lumen of the vessel. All images are representative of observations from a minimum of four mice per staining condition.