Fig. 3: Detection of IFN-γ and correlation between T cell immunity and pre-ART sensitivity to 3BNC117. | Nature Communications

Fig. 3: Detection of IFN-γ and correlation between T cell immunity and pre-ART sensitivity to 3BNC117.

From: Administration of broadly neutralizing anti-HIV-1 antibodies at ART initiation maintains long-term CD8+ T cell immunity

Fig. 3

a Representation of the protocol followed to detect interferon-gamma (IFN-γ) release and ELISpot upon Gag stimulation. b Concentration of IFN-γ (pg/mL) in the supernatants from HIV-1 Gag stimulated PBMCs detected by Mesoscale for each randomization group (n = 13 ART-control group, n = 9 in ART + 3BNC117, n = 9 in ART + RMD and n = 12 in ART + RMD + 3BNC117). Concentration of IFN-γ (pg/mL) in the supernatants (n = 29) (c), frequency of specific-CD8+ T cells (n = 31) from HIV-1 Gag stimulated PBMCs (d) and IFN-γ ELISpot response to Gag (n = 29) (e) and to CEF (CMV, EBV and influenza virus) (n = 29) (f) at 365 days after ART initiation is shown for the following groups: ART-control group, 3BNC117 sensitive individuals receiving the bNAb and 3BNC117 resistant individuals receiving the bNAb. P-values were calculated using two-sided Mann–Whitney test. g Relationship between IFN-γ release after 365 days of ART and pre-ART 3BNC117 IC90 (µg/mL) (n = 16). h Correlation between Gag-specific CD8+ T cell responses after 365 days of ART and % of pre-ART sensitive sequences to 3BNC117 determined by the “bNAb-ReP” algorithm (n = 19). Individuals with no IFN-γ production were assigned a value of 0.1 for inclusion in the graph b and c. R and P-values for two-sided Spearman rank-order correlation are shown in g and h. Source data are provided as a Source Data file.

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