Fig. 1: Immunodominant HLA-A*29:02-restricted T cell immunity in COVID-19 convalescent participants.

SARS-CoV-2 specific T cells from COVID-19 convalescent participants were expanded from PBMC using SARS-CoV-2 encoded antigens. PBMCs were cultured for 2 weeks in the presence of IL-2 and assessed for IFN-g responses following recall with the specific antigen. A Heatmap of the magnitude of SARS-CoV-2 antigen-specific T cell responses in COVID-19-convalescent individuals (HLA-A*29:02⁺, n = 4; HLA-A*29:02^-, n = 55). B Frequency of S-1- and ORF3A-specific IFN-γ-producing CD8⁺ T cells in HLA-A*29:02⁺ and HLA-A*29:02^– participants. Whiskers represent minimum to maximum values, boxes represent the 25th to 75th percentile, line represents the median. C Frequency of IFN-γ-producing CD8⁺ T cells in response to S-1 peptide matrix pools in HLA-A*29:02⁺ participants (n = 2). D Frequency of IFN-γ-producing CD8⁺ T cells in response to the YFPLQSYGF epitope in HLA-A*29:02⁺ participants. E Frequency of IFN-γ-producing CD8⁺ T cells in response to the ORF3A peptide matrix pools in HLA-A*29:02⁺ participants (n = 2). F Frequency of IFN-γ-producing CD8⁺ T cells in response to the YFTSDYYQLY epitope in HLA-A*29:02⁺ participants. G Prevalent HLA-A*29:02 epitope sequences in SARS-CoV-2 variants of concern. H Frequency of IFN-γ-producing CD8⁺ T cells in response to serial dilutions of YFPLQSYGF and YFPLRSYSF in HLA-A*29:02⁺ participants. Data represent mean and standard deviation of duplicates. Source data are provided as a source data file.