Fig. 6: Functional blocking of Cxcr4 and 7 hampered the blood vessel-guided migration.
From: Erratic and blood vessel-guided migration of astrocyte progenitors in the cerebral cortex
a CRISPR vectors against Cxcr4/7 disrupt the association of astrocyte progenitors with blood vessels. b Distances from blood vessels to transfected astrocyte progenitors (GFP+/Aldh1l1+). Cxcr4 CR#1 and #2 significantly increased the distances from the blood vessels, which were enhanced by adding Cxcr7 CR#1 or #2 (two-sided Dunnett’s test, 1767 cells/14 brains control, 725 cells/6 brains Cxcr4 CR#1, 1166 cells/7 brains Cxcr4 CR#2, 936 cells/8 brains Cxcr7 CR#1, 1194 cells/16 brains Cxcr7 CR#2, 557 cells/10 brains Cxcr4 CR#2 + Cxcr7 CR#1, 808 cells/7 brains Cxcr4 CR#1 + Cxcr7 CR#2, 1921 cells/7 brains, control vs. Cxcr4 CR#1, P = 0.0345; control vs. Cxcr4 CR#2, P = 0.0448; control vs. Cxcr4 CR#2 + Cxcr7 CR#1, P = 0.0106; control vs. Cxcr4 CR#1 + Cxcr7 CR#2, P < 0.0001). For detailed information of box plots, see “Statistical analysis” section in Methods. c Glial progenitors transfected with CRISPR vectors were cultured on endothelial tubes, and were pseudo-colored in green and red for those migrating on and off the endothelial tubes, respectively (left). Ratio of cells migrating off versus on the endothelial tubes in each objective field (right, 28 control, 17 Cxcr4 CR#2, and 32 Cxcr7 CR#1 objective fields/3 experiments, Kruskal-Wallis test (P < 0.001) followed by two-sided Dunn’s multiple comparisons test, control vs. Cxcr4 CR#2, P = 0.005; control vs. Cxcr7 CR#1, P < 0001, no significant difference between Cxcr4 CR#2 and Cxcr7 CR#1, P = 0.999). See also Supplementary Movie 15. d Flt1-DsRed mouse embryos were electroporated with CRISPR vectors and employed to time-lapse observations (left panels). CRISPR vectors reduced the migration distance on blood vessels (right panel, 169 control, 63 Cxcr4 CR#2, 72 Cxcr7 CR#1, and 59 Cxcr7 CR#2 cells/3 experiments, two-sided Dunnett’s multiple comparisons test, control vs. Cxcr4 CR#2, P = 0.0089, control vs. Cxcr7 CR#1, P = 0.0015, and control vs. Cxcr7 CR#2, P = 0.0008). See also Supplementary Movie 16. Scale bars, 20 μm (a), 200 μm (c), 50 μm (d). Horizontal bars in scatter plots (c, d) represent mean ± SD. Source data are provided as a Source Data file.
