Fig. 4: Validation immunohistochemistry of the panel of survival markers identified.

Representative fluorescence images of eight proteins in the two OS clusters of patients. Images were acquired with a confocal microscope at ×40 magnification. The experiment was repeated on representative tissues of 23 patients for the prospective cohort and on representative tissues of 50 patients for the validation cohort. For each tissue, 3–4 images were taken for quantification. Scale bar = 20 µm.