Fig. 3: Loss of AR in Gli1-lineage stromal cells impacts the basal epithelial compartment at a single cell resolution.

a Uniform Manifold Approximation and Projection (UMAP) visualization of 10,810 individual cells from Hi-Myc:R26^mTmG/+:Gli1^CreER/+ prostates (top left, gray), 6849 individual cells from Hi-Myc:R26^mTmG/+:Ar^L/Y:Gli1^CreER/+ prostates (top right, dark blue), and integrated cells (bottom). b UMAP representation showing cell-type cluster identities based on gene expression profiles. The dotted line delineates the prostatic epithelial cells. c UMAP plots separated by indicated genotype from b (Far left) and gene expression levels in Hi-Myc:R26^mTmG/+:Gli1^CreER/+ (top) and Hi-Myc:R26^mTmG/+:Ar^L/Y:Gli1^CreER/+ (bottom). Color intensity indicates the scaled expression level in each cell. Pink and green arrows indicate luminal and basal epithelial cell clusters, respectively, and blue and grey arrows indicate stromal fibroblast and smooth muscle cell clusters, respectively. d UMAP visualization of epithelial cells sub-clustered, re-clustered, and labeled by cell type. BE, basal epithelial cells; LE, luminal epithelial cells; OE, other epithelial cells. e Gene expression levels of markers for human Myc transgene (hMycTg), LE (Krt8), and BE (Krt14). f Graph showing the percentage of hMycTg⁺ cells per total basal or luminal cells in each genotype. Source data are provided as a Source Data file. g Representative images of H&E and IHC staining for Myc, cytokeratin 8 (CK8), and cytokeratin 14 (CK14) in adjacent prostate tissue sections of 3-month-old Hi-Myc:R26^mTmG/+:Gli1^CreER/+ (top) and Hi-Myc:R26^mTmG/+:Ar^L/Y:Gli1^CreER/+ (bottom) mice. Scale bars, 20 μm. Red arrows indicate CK14⁺ cells infiltrating towards the lumen. h Colocalization of Myc with either AR, CK8, or CK14 from indicated prostate tissues. Yellow arrows indicate Myc⁺CK14⁺ cells. Scale bars, 20 μm.