Fig. 4: Loss of AR in Gli1-lineage cells reduces IGF and Wnt signaling in basal epithelial cells.

a Gene Set Enrichment Analysis (GSEA) enrichment plots depicting insulin-like growth factor 1 receptor (IGF1R) and Wnt signaling pathways significantly up-regulated in hMycTg⁺ basal epithelial cells in Hi-Myc:R26^mTmG/+:Gli1^CreER/+ versus Hi-Myc:R26^mTmG/+:Ar^L/Y:Gli1^CreER/+. See also “Methods” section. NES, normalized enrichment score; FDR, false discovery rate. b Box plots representing scaled expression data for IGF1R (top) or Wnt (bottom) downstream genes in hMycTg⁺ basal cells from indicated genotypes (HiMyc, n = 1363; HiMyc-ARKO, n = 36). Pink lines mark the median; top and bottom lines of boxes indicate the boundaries of the first and third quartiles, respectively; the top and bottom whiskers show the maximum and minimum values, respectively, excluding outliers. c Heatmap of pairwise Spearman correlation between the indicated gene expression in hMycTg⁺ basal cells from indicated genotypes. Colors reflect the level of the correlation coefficient. Numbers show the correlation coefficient. d qPCR analysis of gene expression as indicated in the figure shown as fold change in sort-purified basal cells from indicated genotypes. Data are represented as mean ± SD of three biological replicates. Two-sided Student’s t test, ^*p < 0.05, ^**p < 0.01. The exact p values and related source data are provided as a Source Data file. e Representative images of triple-immunofluorescent (IF) staining for indicated antibodies on prostate tissue sections from Hi-Myc:R26^mTmG/+:Gli1^CreER/+ (top) and Hi-Myc:R26^mTmG/+:Ar^L/Y:Gli1^CreER/+ (bottom). Yellow arrows indicate triple-positive stained cells. Scale bars, 25 μm.