Fig. 3: Reduced endothelial Cav-1 facilitates vascular HSP90α secretion.
a–d Immunofluorescent images, immunoblotting and quantifications showing the changes of Cav-1 in BMECs of control and CoCl2 group. Results represent the mean for the relative band intensity of five replicates. Scale bar, 20 μm. (e) Volcano plot showing highly expressed secretory factors by endothelial cells treated with chronic hypoxia. HSP90α (red spot) is shown as a highly expressed secretory protein compared to control. Spots of p value <0.05 with log2 [fold change] >0.5 or < −0.5 were considered as potential proteins to be chosen and represented in blue. Grey spots showing no differences between two groups (n = 4 independent primary cell cultures/group). f ELISA displaying the amount of HSP90α in control CM and CoCl2 CM (n = 5 independent primary cell cultures/group). g–i Immunostaining of Cav-1, HSP90α, and CD31 and quantifications showing the decreased Cav-1 expression and elevated HSP90α secretion in BCAS mice (n = 5 mice/group). Scale bar, 20 μm. j–m Immunoblotting and quantifications of Cav-1 level in microvascular segments and HSP90α level in the CC brain tissue. Results represent the mean for the relative band intensity of five replicates. For immunoblotting experiments, protein samples derived from the same experiment and gels/blots were processed in parallel. All data are presented as the mean ± SD. The data in b, d were compared by one-way ANOVA with Tukey post hoc test. The data in others were compared by paired t-test. Source data are provided as a Source Data file.
