Fig. 4: Over-expression of endothelial Cav-1 inhibits HSP90α secretion and attenuates ischemic demyelination.

a Experimental flow chart. b, c Representative fluorescent images and qualification of the transfection of GFP (green) reporter AAV into microvessels (CD31⁺, red) with enforced Cav-1 (light blue) expression in the CC of BCAS mice (n = 5 mice/group). Scale bar, 20 μm. d–f Double immunostaining and qualifications of CD31 and HSP90α showing the reduction of HSP90α induced by AAV-Tie1-Cav-1 (n = 5 mice/group). Scale bar, 20 μm. g Representative images showing the PDGFRα⁺ clusters contacting vessels (n = 5 mice/group). Scale bar, 20 μm. h, i Quantifications of cluster number and area in BCAS mice treated with control AAV or AAV-Tie1-Cav-1 (n = 5 mice/group). j–l Representative immunofluorescence images and quantifications showing the changes in the percentage of BCAS1⁺ newly formed immature oligodendrocytes and CC1⁺Olig2⁺ mature oligodendrocytes (n = 5 mice/group). Scale bar, 20 μm. m, n Representative MBP and MAG staining and quantifications (n = 5 mice/group). Scale bar, 20 μm. All data are presented as the mean ± SD. The data were compared by paired t-test. Source data are provided as a Source Data file.