Fig. 5: HSP90α siRNA rescues white matter abnormality in wild type and Cav-1−/− BCAS mice. | Nature Communications

Fig. 5: HSP90α siRNA rescues white matter abnormality in wild type and Cav-1−/− BCAS mice.

From: Vascular endothelium deploys caveolin-1 to regulate oligodendrogenesis after chronic cerebral ischemia in mice

Fig. 5

a Flow chart. b Representative images of Cy5-siRNA (red) uptake in CD31+ vessels (grey) with HSP90α (green) in the CC of wild type and Cav-1−/− mice (n = 5 mice/group). Scale bar, 20 μm. c Quantification of endothelial and secretory HSP90α intensity (normalized to CD31 intensity) (n = 5 mice/group). d Representative staining and reconstruction images of PDGFRα+ OPC clusters attached on the vessel (n = 5 mice/group). Scale bar, 20 μm. e, f Quantification of frequency and sizes of perivascular clusters (n = 5 mice/group). g–i Representative images and quantifications of newly-formed pre-myelinating cells (BCAS1+ or ENPP6+). j, k Representative images, and quantifications showing the changes in mature oligodendrocytes (CC1+Olig2+) (n = 5 mice/group). Scale bar, 20 μm. All data are presented as the mean ± SD. The data were compared by one-way ANOVA with Tukey post hoc test. Source data are provided as a Source Data file.

Back to article page