Fig. 6: Endothelial miR-3074-1-3p targeting Cav-1 is upregulated after BCAS. | Nature Communications

Fig. 6: Endothelial miR-3074-1-3p targeting Cav-1 is upregulated after BCAS.

From: Vascular endothelium deploys caveolin-1 to regulate oligodendrogenesis after chronic cerebral ischemia in mice

Fig. 6

a–c Systematic screening of upregulated miRNAs targeting Cav-1 after 4 weeks of BCAS (n = 4 mice/group). d, e Verification of 13 miRNAs in the CC of BCAS mice (n = 6 mice/group) and BMECs under chronic hypoxia (n = 6 independent primary cell cultures/group). f, g Combined FISH and immunofluorescence labeling of miR-3074-1-3p and Cav-1 in BMECs of control and CoCl2 groups (n = 5 independent primary cell cultures/group). Scale bar, 50 μm. h, i Dual luciferase reporter assay of the interaction of miR-3074-(1)-3p mimic and wild type mouse/human Cav-1 (n = 5 independent primary cell cultures/group). j, k Immunoblotting and quantification showing the Cav-1 level in BMECs transfected with agomir or antagomir. Results represent the mean for the relative band intensity of five replicates. For immunoblotting experiments, protein samples derived from the same experiment and gels/blots were processed in parallel. All data are presented as the mean ± SD. The data in k were compared by one-way ANOVA with Tukey post hoc test. The data in others were compared by paired t-test. Source data are provided as a Source Data file.

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