Fig. 8: FGFR2b recycling regulates autophagy and the balance between proliferation and cell death.
a Autophagy measured by acridine orange staining of HeLa_FGFR2bST (left) or T47D (right) transfected either with wtRAB11, DnRAB11 or DnDNM2 and incubated or not with FGF10 for 2 h. N = 3 independent biological replicates where at least six treated wells of cells were counted. p value ≤ 0.001*** (one-way ANOVA with Tukey test). b Immunoblot analysis (N = 3 independent biological replicates) with the indicated antibodies of HeLa_FGFR2bST transfected either with GFP or DnRAB11 and treated with vehicle (UT) or with FGF7 and FGF10 for different time points. Measurement of autophagy by acridine orange staining (c), cell proliferation by EdU incorporation (d) and apoptosis by cleaved caspase 3 activated dye (e), in T47D treated with the FGFR inhibitor (FGFRi: PD173074), ULK1 inhibitor (ULKi: ULK101), ULK1/2 inhibitor (ULK1/2i: SBI0206965) or mTOR inhibitor (mTORi: Rapamycin), stimulated or not with FGF10 for 2 h. Data were presented as percentage compared to untreated cells. N = 3 independent biological replicates where at least six treated wells of cells were counted. p value ≤ 0.001*** (one-way ANOVA with Tukey test). f Model of FGFR2b global and proximal signalling partners during recycling to the plasma membrane. Long-term responses are indicated based on the data of this study. The black arrow indicates FGFR2b trafficking. The green arrow indicates events activated by FGFR2b regardless of its subcellular localisation. Source data are provided as a Source Data file.
