Fig. 2: TaMTB positively regulates WYMV infection.
From: N6-methyladenosine RNA modification promotes viral genomic RNA stability and infection
a The accumulation of WYMV RNA1 and RNA2 in the WYMV-infected TaMTB transgenic (TaMTB-OE) or wild type (WT) plants as determined by qPCR using CP and P2 gene-specific primers. #1, 3 are two independent transgenic lines of TaMTB-OE. Values are means ± SD (two-sided t test, n  =  3, P  =  0.0011, 0.0045, 0.0085, 0.0025, respectively). **P  <  0.01. b Detection of WYMV CP accumulation in WT or two TaMTB-OE lines plants by western blot using a CP-specific antibody. Three times each experiment was repeated independently with similar results. c Assessment of TaMTB-OE plants for disease resistance in a virus-contaminated nursery at Yangzhou, Jiangsu Province in 2022. WT represents Fielder plants. d WYMV RNAs accumulation in wheat plants co-infected with WYMV and BSMV:TaMTB or WYMV and BSMV:00. Total RNA from BSMV:00 and WYMV co-infected plants (BSMV:00) were used as negative controls. Values are means ± SD (two-sided t test, n  =  3, P  =  0.0128, 0.0189, respectively). *P  <  0.05. e Detection of WYMV CP accumulation in WYMV-infected BSMV:00 and BSMV: TaMTB plants by western blot using a CP-specific antibody. Three times each experiment was repeated independently with similar results. f Phenotypes in the fourth leaves of the plants inoculated with phosphate buffered saline (Mock), BSMV, WYMV, BSMV:PDS, BSMV + WYMV and BSMV:TaMTB+WYMV, respectively. Ponceau staining (Ponceau S) shows equal protein loadings in each lane. TaCDC was used as the internal control gene. Source data are provided as a Source Data file.
