Fig. 7: Mutation of SNP176A/C in TaMTB promotes viral infection.
From: N6-methyladenosine RNA modification promotes viral genomic RNA stability and infection
a Gene structure display and DNA polymorphisms of TaMTB-B1. The black box represents the CDS region, the blank space represents the UTR region. Different colors represent different types of bases. b Comparison of WYMV resistance between wheat varieties carrying Hap 1-4 genotypes. The infection type is derived from the mean value of all replicates. n represent the number of wheat accessions with the corresponding haplotype. Statistics: for both datasets, two-sided t test was performed, P =  0.0014, **P  <  0.01. c Binding affinity of TaMTB(SNP176A) or TaMTB(SNP176C) with NIb detected by microscale thermophoresis (MST) experiment. The mixture with TaMTB(SNP176C) + His was used as a negative control. The solid curve is the fit of the data points to the standard Kd-Fit function. Each binding assay was repeated three times independently, and bars represent SD. Data points are represented as means ± SD. d m6A-IP-qPCR assay to detect the m6A level of peak 3 in WYMV-infected wheat plants which transient expressed TaMTB(SNP176A) or TaMTB(SNP176C). Values are means ± SD (two-sided t test, n  =  3, P =  0.01) **P  <  0.01. e The accumulation of WYMV RNA1 and RNA2 in WYMV-infected wheat plants which transient expressed TaMTB(SNP176A) or TaMTB(SNP176C) were determined by qRT-PCR using CP and P2 gene-specific primers. Values are means ± SD (two-sided t test, n  =  3, P =  0.0335, 0.0248) *P  <  0.05. f The mRNA lifetimes of WYMV CP. Data are represented as means ± SD for 3 biological replicates (two-sided t test, P  = 0.0172). TI: transcription inhibition. g m6A-IP-qPCR assay to detect the m6A level of CP mRNA in N. benthamiana leaves co-expressed with 35 S:CP-GFP + TaMTB(SNP176A) or 35 S:CP-GFP + TaMTB(SNP176C). Values are means ± SD (two-sided t test, n  =  3, P =  0.0419) *P  <  0.05. Source data are provided as a Source Data file.
