Fig. 3: scLCDV1-VILP is a potent IGF1R antagonist.
From: Interaction of a viral insulin-like peptide with the IGF-1 receptor produces a natural antagonist
Western blot detection of IGF1R, IR, AKT, ERK1 and ERK2 phosphorylation in lysates of murine preadipocyte overexpressing the human IGF1R (a) or the human IR isoform B (b) as described in Methods. Quantitation of phosphorylated IGF1R, IR, AKT and ERK1. Scan intensities were normalized to total protein, and data expressed as mean ± SEM (two-way ANOVA followed by a Šídák’s multiple comparison test; Graphpad Prism V.9; n = 4 (IGF1R) and 6 (IR) independent experiments; data were normalized by phosphorylation intensity induced by 10 nM of IGF-1 (c) or insulin (d).
