Fig. 4: scLCDV1-VILP reduces cell proliferation and growth in mice oversecreting bovine growth hormone.

a Murine preadipocytes overexpressing the human IGF1R were plated at a density of 20,000 cells/well then counted after a 48-h incubation with the indicated concentrations of IGF-1, scLCDV1-VILP and dcLCDV1-VILP. Data are expressed as the mean ± SEM (Two-way ANOVA followed by a Dunnett’s multiple comparison test (Graphpad Prism V.9); n = 3 independent experiments). b The number of murine preadipocytes expressing the hIGF1R were counted after a 48-h incubation with various concentrations of scLCDV1-VILP and dcLCDV1-VILP and a fixed concentration of IGF-1 (10 nM). Data are expressed as the mean ± SEM (two-way ANOVA followed by a Šídák’s multiple comparison test (Graphpad Prism V.9); n = 3 independent experiments). c Viable murine preadipocytes overexpressing the hIGF1R were stained with crystal violet after a 48-h incubation with various concentrations of IGF-1, scLCDV1-VILP and dcLCDV1-VILP. Data are express as mean ± SEM (ns, not significant; Kruskal-Wallis test followed by a Dunn’s multiple comparisons test (Graphpad Prism V.9); n = 5 (scLCDV1-VILP) and 6 (dcLCDV1-VILP) independent wells). d Relative expression of the LCDV-1 and IGF-1 mRNA expression in liver samples from mice that received either an AAV control (n = 6 mice) or the AAV LCDV-1 (n = 8 mice). Data are expressed as the mean ± SEM (ns, not significant; two-sided Mann–Whitney test; Graphpad Prism V.9). e Body weight gain at week 2 and 6 following AAV injection. Data are normalized by the basal weight measured at t = 0 and expressed as mean ± SEM (two-sided Mann–Whitney test; Graphpad Prism V.9; AAV control = 6 mice, AAV LCDV-1 = 8 mice). f The increment (delta) of body weight gain was measured weekly for seven weeks following the AAVs injection. Data are express as mean ± SEM (AAV control=6 mice, AAV LCDV-1 = 8 mice). g Fasting blood glucose was assessed 2 and 5 weeks after the AAVs injection. Data are express as mean ± SEM (ns, not significant; two-way ANOVA followed by a Šídák’s multiple comparison test; Graphpad Prism V.9; AAV control=6 mice, AAV LCDV-1 = 8 mice).