Fig. 5: Molecular patterns of splicing factors in immune cell-type-specific gene expression or multisite proteomes and their relationship with metastasis.
From: Connecting multiple microenvironment proteomes uncovers the biology in head and neck cancer
a Expression of the six selected targets (SRSF1, SRSF2, SRSF3, SRSF5, TRA2A, and CD209) in immune populations from HNSCC that were identified by scRNASeq public data5 in lymph node pN+ microenvironment. b Average fold change (y axis) and adjusted p (x axis) between pN+ and pN0 tumor microenvironments5 for the six targets evaluated in individual immune populations (two-sided Wilcoxon test followed by Benjamini-Hochberg correction). The dashed lines represent a p threshold of 0.05 and significant results are indicated by arrows. The differences could not be tested for plasma and CD8+ T exhausted cells because there were not enough cells for comparison. c Heat-map representing the average fold change for GO splicing genes differentially expressed between pN+ and pN0 immune populations from tumor microenvironments5 (pN+ vs. pN0; adjusted p ≤ 0.05; two-sided Wilcoxon test followed by Benjamini-Hochberg correction). Blank cells indicate transcripts without difference between pN+ and pN0. d Proportion of up- and downregulated genes from (c) that are expressed by the immune cells. Gene expression (up- and downregulation in pN+ when compared to pN0) was significantly associated with the immune cell types (p = 0.0210; two-sided Fisher’s exact test). *p ≤ 0.05. e Heat-map representing the average ratio for GO splicing proteins differentially expressed between pN+ and pN0 multisites (pN+ vs. pN0; p ≤ 0.05; two-sided unpaired Student’s t-test). The log2 LFQ ratios are shown for the statistically significant proteins. Blank cells indicate proteins that were not differentially abundant between pN+ and pN0. f Proportion of up- and downregulated proteins from (e) expressed in the multisites. Protein abundance (up- and downregulation in pN+ when compared to pN0) was significantly associated with the environments (p = 0.0008; two-sided Fisher’s exact test). LN: lymph node; PT: primary tumor. ***p ≤ 0.001. Source data are provided as a Source Data file.
