Fig. 3: The tRNA anticodon loop passes through a molecular RY-gate in ADAT2. | Nature Communications

Fig. 3: The tRNA anticodon loop passes through a molecular RY-gate in ADAT2.

From: Structural basis for sequence-independent substrate selection by eukaryotic wobble base tRNA deaminase ADAT2/3

Fig. 3

a Superposition of the tRNA-bound gate in TbADAT2/3 and the apo-gate in MmADAT2/3 (PDB: 7NZ7). The side chain of R159TbADAT2 is represented as flexible due to its poor side chain density. b The corresponding positions in bacteria deaminase TadA, RNA bound versus unbound (PDB: 2B3J and 1WWR, respectively). c Fold change of the observed rate constants of mutants to wild-type (krel = kobs mutant/kobs WT). Graph values calculated from three independent replicates. All mutants are of TbADAT2 co-expressed with TbADAT3. n.d. denotes no detectable activity after 24 h assay incubation. kobs values obtained from single-turnover kinetic assays (n≥3). Source data are provided as a Source Data file.

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