Fig. 5: ADAT3 N-terminal domain probes the three-dimensional structure of the tRNA.
a Two views of the ADAT3N fitted in the post-processed EM map, in blue, with ‘A’ and ‘B’ regions as the two contact points of our model with tRNA, as indicated in C. b ADAT3N including linkers (residues 4–172); surface model colored by vacuum electrostatic potential, positive charges in red and negative charges in blue, with D-Arm of tRNA in a black cartoon representation. Residues identified to play a role in tRNA binding are annotated in black, in olive from41 and in yellow from40. (c) Schematic of the tRNA with interaction contacts. Anticodon-stem loop interactions are represented as a summary of the ones indicated in Fig. 2D. The two ADAT3N contact points, ‘A’ and ‘B’ are indicated in blue as above.
