Fig. 3: Preparation of ARNGs@TMZ/ICG and NIR-induced deformation.

a Schematic illustration of camouflaging ARNGs@TMZ/ICG by ApoE peptide-decorated erythrocyte membranes and NIR-induced deformation of the nanogels. b, c Size distribution (b) and zeta potential (c) of various nanoparticles measured by DLS (n = 3 independent experiments). Data are presented as mean ± SD d, e TEM images of ARNGs@TMZ/ICG before (d) and after (e) NIR irradiation (808 nm, 0.5 W cm⁻², 5 min). f Particle size distribution of ARNGs@TMZ/ICG with and without NIR irradiation (808 nm, 0.5 W cm⁻², 5 min). g Young’s modulus of ARNGs@TMZ/ICG after receiving different time of NIR irradiation (808 nm, 0.5 W cm⁻²) measured by AFM (n = 3 independent samples). Data are presented as mean ± SD (one-way ANOVA and Tukey’s multiple comparison test). h, i In vitro release profile of TMZ (h) and ICG (i) from ARNGs@TMZ/ICG in pH 7.4 phosphate buffer at 37 °C, with and without NIR irradiation (808 nm, 0.5 W cm⁻²) (n = 3 independent experiments). Data are presented as mean ± SD.